Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm

Abstract Background Leaf color mutants are the ideal materials to explore pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai ( Brassica campestris L.) mutant “WY16–13” was identified, which exhibited during its entire growth period. However, current understanding molecular mechanism underlying Chl metabolism development is limited. Results Total carotenoid content in WY16–13 reduced by 60.92 58.82%, respectively, as compared with wild type parental line W16–13. Electron microscopic investigation revealed fewer chloroplasts per cell looser stroma lamellae than A comparative transcriptome profiling performed using leaves from (WY16–13) normal green-leaf (W16–13). total 54.12 million (M) 56.17 M (W16–13) reads were generated. 40,578 genes identified mapped libraries. We 3882 differentially expressed (DEGs) W16–13 (i.e., 1603 upregulated 2279 downregulated genes). According Gene Ontology (GO) term Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses, these DEGs involved porphyrin [i.e., chlorophyllase CLH ), heme oxygenase HO (ide) b reductase NYC protochlorophyllide oxidoreductase POR ) genes], carbohydrate photosynthesis, carbon fixation photosynthetic organisms. Moreover, deficiency biosynthetic intermediates that formation phenotype related disorder metabolism. Conclusions Our results provide valuable insights into bioinformatics resource for further functional identification key allelic responsible differences content.